BIOLOGICAL STUDIES ON FORMYCIN AND FORMYCIN B

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Metabolic conversion of formycin B to formycin A and to oxoformycin B in Nocardia interforma.

A strain of Nocardia interforma having low productivity of formycin A was selected. Washed cells of this strain convert formycin B to oxoformycin B (reaction I), while those of the original strain collected during formycin A producing phase convert formycin B to formycin A (reaction II). Reaction I is inhibited by allopurinol, an inhibitor of xanthine oxidase. Reaction II is inhibited by hadaci...

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Biochemical studies of the nucleoside analogue, formycin.

The absorption and fluorescence properties of formycin provide a useful tool in the analysis of enzyme-substrate interactions; we have therefore studied selected biochemical properties of formycin nucleotides. Formycin and its derivatives effectively substitute for the corresponding adenosine substrates in a wide variety of enzyme reactions. These include enzymes of nucleotide metabolism, RNA p...

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Biochemical effects of formycin B on Xanthomonas oryzae.

Formycin B, an inosine analogue, inhibits the incorporation of radioactive adenosine, uridine and thymidine, but none of formic acid, orotic acid and inorganic phosphoric acid into the cellular nucleic acid of Xanthomonas oryzae. The biochemical effects are readily reversed when formycin B is removed. Cell-free extract from the organism is entirely insensitive to formycin B in respect to phosph...

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Biosynthesis of the formycin family.

The formycin family is composed of the G-ribosides of pyrazolopyrimidine derivatives1'^. In a previous paper we proposed a scheme for their biosynthesis in Nocardia interforma as follows :? ->formycin B monophosphate (FMBP)-» formycin A monophosphate (FMAP) -> formycin A (FMA) -* formycin B (FMB) -> oxoformycin B3). This schemeonly explains the terminal steps of the biosynthetic pathway while t...

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Biosynthesis of formycin. Incorporation and distribution of labeled compounds into formycin.

1. Two carbons, carbon-2 and one of carbons-3 to -5, of lysine seemed likely to be incorporated into one of carbon of the chromophore moiety of formycin. 2. From the results of radioisotopic studies with glutamate, gamma-amino-n-butyrate or organic acids related to tricarboxylic acid cycle, the important role of glutamate in the biosynthesis of formycin was strongly suggested. 3. The incorporat...

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ژورنال

عنوان ژورنال: The Journal of Antibiotics

سال: 1968

ISSN: 0021-8820,1881-1469

DOI: 10.7164/antibiotics.21.5